Category: Laboratory Research
Acute Hepatotoxicity of Dentin Bonding Agents in ICR Mice
Jeongwon Oha* (wwwonny@gmail.com) | Hyunjung Koa | Miri Kima
aAsan Medical Center, University of Ulsan, Seoul, Republic of Korea
Objectives: There are few studies on the potential toxicity of bonding agents despite their possibility of accidental ingestion during restorative procedures. The aim of this study was to evaluate the acute hepatotoxicity of orally delivered dentin bonding agents in ICR mice.
Methods: All five groups were prepared based on the type of the dentin bonding agents: saline (negative control), acetaminophen (500mg/kg, positive control), total-etch adhesive (TA, 5ml/kg, Scotchbond™ Multi-Purpose, 3M, USA), self-etch adhesive (SA, 5ml/kg, Clearfil SE Bond, Kuraray, Japan) and universal adhesive(UA, 5ml/kg, All Bond Universal, BISCO, USA).
Fourteen female ICR mice were randomly divided into 5 groups. Acetaminophen was dissolved in physiological saline, and all samples were dosed by intragastric gavage daily for 7 days. After 7 days, all animals were sacrificed. Blood samples were obtained and the serum marker enzymes – AST (aspartate aminotransferase), ALT (alanine aminotransferase) and ALP (alkaline phosphatase) – were estimated. Liver sections from each group were prepared for histopathological analysis and evaluated by microscope (Olympus DP73, Japan). Comparison of biochemical levels was carried out using one-way ANOVA (α=0.05).
Results: None of the groups experienced significant elevation of the serum marker enzymes (AST, ALT, ALP) beyond the known-normal range of ICR mice. However, the histological results showed that all groups exhibited significant inflammatory changes, such as mononuclear cellular infiltration, vascular congestion, and hepatocyte swelling. Histopathologically, the SA and UA group expressed moderate, and the TA group expressed mild inflammatory reactions.
Conclusion: The biochemical alterations of orally administrated dentin bonding agents were not significant, but histopathological findings revealed the presence of inflammatory signs of liver in all experimental groups. This suggests that the unintentional ingestion of dentin bonding agents may cause acute liver inflammation. Practitioners should take care to prevent accidental intake of bonding agents during restorative procedures.
Funding/Conflict of Interest: None
Keywords: chemically-induced liver toxicity, dentin-bonding agents, ICR mice
