Cytotoxic Effect of TEGDMA on Healthy and Inflamed Pulp Stem Cells

Sebahat Melike Durukan^a^* (melike.durukan@altinbas.edu.tr) | Servet Özcan^b

^aAltinbas University, Istanbul, Türkiye

^bErciyes University, Kayseri, Türkiye

Objective: This study aims to look at how TEGDMA affects pulpal stem cells derived from healthy teeth and pulpal stem cells derived from teeth affected by caries.

Methods: Senescence, apoptosis, and cell proliferation tests were meticulously performed on healthy and carious tooth-derived dental pulp stem cells (DPSCs) after treatment with TEGDMA resin monomer on the cells. Data were subjected to two-way ANOVA test.

Results: We observed that caries DPSCs treated with high doses of TEGDMA showed an enhanced susceptibility to cell senescence among our TEGDMA-treated research groups, which we summarized as follows: Similar results were obtained for necrosis and apoptosis. Significant differences were found according to the ANOVA (p<0.05).

Conclusion: Future research should focus on the long-term biological impacts of adhesive compounds on carious DPSC, according to our findings. The most noteworthy outcome of our research was its contribution to the biological knowledge of the necrosis process in carious teeth treated with dentin bonding agents, which was an important breakthrough.

Funding/Conflict of Interest: The investigation is funded by Ethics Committee of Erciyes University Clinical Research Ethics Committee decision numbered 2019/573 and dated 24.07.2019. The samples used in the study were obtained from Erciyes University Faculty of Dentistry.

Keywords: adhesive agent, apoptosis, cytotoxicity, dental pulp stem cell, senesence, TEGDMA

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