Effect of Phytic Acid on the Efficacy of Bioactive Desensitizers

Manoj Sindagi Swabhaanu^a* (swabhaanu.sindagi@gmail.com) | Paramashivaiah Ashwini^a | Mehta Deepak^b

^aRajiv Gandhi University of Health Sciences, Bangalore, India

^bTohuku University, Sendai, Japan

Objective: The objectives of this in-vitro study were to evaluate dentin tubule occlusion caused by Predicta™ Bioactive Desensitizer, a methacrylate-free, nanohydroxyapatite-based desensitizer, and the effects of phytic acid pretreatment.

Methods: Occlusal surfaces were prepared from 55 caries-free human premolars extracted as part of orthodontic treatment with informed consent and were stored in .5% thymol solution at 4°C and then randomly allocated to 6 groups each consisting of 10 samples except the control group. Using a low-speed diamond saw, 2 mm thick dentin discs and the central area with a diameter of 5 mm was defined as the experimental area and polished with 600 and 1000 grit silicon carbide. These polished samples were immersed in 17% ethylenediaminetetraacetic acid (EDTA) for 40 min to open the dentin tubules and the residual smear layers were removed with distilled water wash; then ethanol-facilitated dehydration for 30 min. Groups A, B, C, D and E (n=10 each) were treated with Predicta™ Bioactive Desensitizer. Samples of Group D and E were pretreated with 1% phytic acid with and without wash respectively; Group F (n=5) was left immersed in artificial saliva as a control. Micromorphological analysis using a scanning electron microscope was performed to assess tubular occlusion. A blinded reviewer independently scored the level of tubule occlusion. Means were compared using one-way analysis of variance (ANOVA). Post-hoc comparisons were made using Tukey test. A 2-sided test with α=0.05.

Results: The mean occlusion scores computed from micromorphological analysis using scanning electron microscope showed that; Group E>Group D>Group C=Group D>Group A>Group F.

Conclusion: The tubule occlusion efficacy of Predicta Bioactive Desensitizer seems to be more effective with 1% phytic acid pretreatment than without.

Funding/Conflict of Interest: None

Keywords: dentin, tubuli occlusion, dentin sensitivity, phytic acid

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