Category: Laboratory Research
Effect of Myristyl Trimethyl Ammonium Bromide on the Physicochemical and Biological Properties of an Experimental Adhesive Resin
Vicente Leitunea* (vicente.leitune@ufrgs.br) | Gustavo Huertas Mogollónb | Isadora Garciac | Fernanda Visiolia | Fabrício Colaresa
aUniversidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
bUniversidad Científica Del Sur, Lima, Peru
cUniversity of Maryland School of Dentistry, Baltimore, MD, USA
Objectives: This study aimed to evaluate the effect of the addition of myristyl trimethyl ammonium bromide (MYTAB) on the physicochemical and biological properties of an experimental adhesive resin.
Methods: The adhesive resin was formulated with methacrylate monomers and a photoinitiator system. The quaternary ammonium compound MYTAB was added at 0.5 (G0.5%), 1 (G1%), and 2 (G2%) wt.% in the adhesive resin. A group without the addition of this compound was used as a control (GCtrl). The resins were evaluated for polymerization kinetics and degree of conversion (n=3), ultimate tensile strength (n=5), contact angle and surface free energy (n=5), cytotoxicity (n=5), and antibacterial activity against Streptococcus mutans considering the biofilm formation (n=3) and planktonic bacteria (n=3).
Results: The polymerization kinetics were similar between all groups and there was no difference regarding ultimate tensile strength too (p>0.05), but there was difference only between G0.5% and G1% for degree of conversion. There was no difference between groups for the water contact angle (p>0.05). G2% had the highest contact angle value with αbromonaphthalene (p<0.05), but there was no difference between groups regarding surface free energy (p>0.05). The cell’s viability ranged from 90.43% (±3.35) for GCtrl to 40.79% (±8.44) for G2% (p<0.05), without statistical difference between GCtrl and G0.5% (p>0.05). All groups with MYTAB showed higher antibacterial activity against biofilm formation and planktonic bacteria than control group (p<0.05).
Conclusion: The addition of 0.5% of MYTAB induced antibacterial activity for the adhesive without affecting physicochemical and biological properties.
Funding/Conflict of Interest: This study was partially funded by CAPES - Finance Code 001 Scholarship.
Keywords: antibacterial agent, adhesive, polymerization, tensile strength, contact angle, Streptococcus mutans
