Category: Laboratory Research
Dentin Bond Strength and Monomer Conversion of Adhesive Containing Flavonoids
Beatriz O. Sahadia | Carolina B. Andréb | Marina R. Santia | Maicon Sebolda | Tainah O. Rifanea | Vitaliano G. Araújo-Netoa | Vicente C. B. Leitunec | Marcelo Gianninia* (gianinni@unicamp.br)
aPiracicaba Dental School, University of Campinas, Piracicaba, Brazil
bSchool of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Brazil
cSchool of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
Objectives To evaluate the effects of three flavonoids added to a commercial universal adhesive on the dentin bond strength (DBST) after 24 h or one-year of water storage. Additionally, the influence of the incorporations of such flavonoids on the degree of conversion (DECO) of the adhesive was determined.
Methods: Alcoholic solutions of 20 mM of baicalein (BA), naringin (NA) and kaempferol (KA) were added to an adhesive (Clearfil Universal Bond Quick, Kuraray Noritake). The control was the same adhesive without flavonoids. Experimental groups (BA, NA and KA) and the control were applied to moist, etched dentin (35% phosphoric acid for 15 s) for the microtensile DBST test (n=8). Restored human molar teeth from the Control and experimental groups were sectioned to obtain specimens with approximately 1.0 mm2 in cross-section. Specimens were tested after 24 h or one-year of water storage using a universal testing machine (0.5 mm/min, until failure). The DECO was measured using Fourier-transform infrared spectroscopy and calculated using standard techniques of observing changes in aliphatic-to-aromatic peak ratios before and after 10 min of initiation of light-activation (n=3). DBST data were analyzed by two-way ANOVA and Tukey’s test, while the DECO was analyzed by two-way ANOVA (α=0.05).
Results: The Control presented the lower DBST than those obtained for NA, BA and KA, at both evaluation times. The DBST reduced after 1-year storage for the Control and KA. The addition of flavonoids did not reduce the DECO.
Conclusion: The incorporation of flavonoids into the adhesive did not negatively influence the DECO. However, they yielded a significant increase in DBST with stability after one year of storage in water, depending on the type of flavonoid.
Funding/Conflict of Interest: FAPESP 2021/11972-0
Keywords: adhesives, micro tensile bond Strength, degree of conversion, dentin, flavonoids
